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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
RhoA G-LISA Activation Assay (absorbance)
RhoA G-LISA Activation Assay (absorbance) from Cytoskeleton, Inc.
Be the first to write a review!
Citations:
(118)
Supplier Page
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Cytoskeleton, Inc. for
RhoA G-LISA Activation Assay (absorbance)
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Product Specs
Item
RhoA G-LISA Activation Assay (absorbance)
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
BK124
Detection Target
RhoA
Quantity
KIT 96 assays
Method
Rho G-LISA Assay Kits
Applications
ELISA
Brand/Trademark
Cytoskeleton, Inc
Add to Compare List
Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (118)
(1)
Key role for Rac in the early transcriptional response to extracellular matrix stiffness and stiffness-dependent repression of ATF3
Journal of cell science
October 1, 2023
Dang I, Brazzo JA, Bae Y, Assoian RK.
Rho activity was assessed in duplicate using the G-LISA small G-protein activation
assay
kit (
BK128
,
BK124
,
Cytoskeleton
) according to the manufacturer's instructions. Briefly, serum-starved MEFs were replated
(2)
The COPD GWAS gene ADGRG6 instructs function and injury response in human iPSC-derived type II alveolar epithelial cells
The American Journal of Human Genetics
October 1, 2023
Rhiannon B. Werder, Kayleigh A. Berthiaume, Carly Merritt, Marissa Gallagher, Carlos Villacorta-Martin, Feiya Wang, Pushpinder Bawa, Vidhi Malik, Shawn M. Lyons, Maria C. Basil,
et al.
iAT2s, 7 days post passage. RhoA activity was measured using the RhoA G-LISA Activation
Assay
Kit (
BK124
,
Cytoskeleton
), with protein normalized prior to running the
assay
, as per manufacturer’s instructions.
(3)
Ouabain Induces Transcript Changes and Activation of RhoA/ROCK Signaling in Cultured Epithelial Cells (MDCK)
Current Issues in Molecular Biology
September 14, 2023
Jacqueline Martínez-Rendón, Lorena Hinojosa, Beatriz Xoconostle-Cázares, José Abrahán Ramírez-Pool, Aída Castillo, Marcelino Cereijido, Arturo Ponce
treatments. The level of GTP-loaded RhoA was measured with G-Lisa
Assay
Biochem Kit (
Cytoskeleton
, Cat.
BK124
, Denver, CO, USA) in samples derived from control and ouabain-treated cells (ouabain 10 nmol/L, 1 h).
(4)
LRRC8A anion channels modulate vascular reactivity via association with Myosin Phosphatase Rho Interacting Protein (MPRIP)
The FASEB Journal
August 10, 2023
Hyehun Choi, Michael R. Miller, Hong‐Ngan Hong-Ngan, Jeffrey C. Rohrbough, Stephen R. Koch, Naoko Boatwright, Michael T. Yarboro, Rajan Sah, W. Hayes McDonald, J. Jeffrey Reese,
et al.
for 3 min before the
assay
. RhoA activity was assessed using the G-LISA RhoA activation assay kit (#
BK124
,
Cytoskeleton, Inc
., Denver, CO) according to the manufacturer’s specifications.
MPRIP
Sulfenylation A
(5)
AraC interacts with p75NTR transmembrane domain to induce cell death of mature neurons
Cell Death & Disease
July 17, 2023
Vanessa Lopes-Rodrigues, Pia Boxy, Eunice Sim, Dong Ik Park, Michael Habeck, Josep Carbonell, Annika Andersson, Diana Fernández-Suárez, Poul Nissen, Anders Nykjær,
et al.
30 min. RhoA activity was evaluated in total CGNs extracts using the RhoA G-Lisa kit (
Cytoskeleton
;
BK124
) following the manufacturer’s instructions. An equal amount of protein was used from each sample as
(6)
A YAP/TAZ-ARHGAP29-RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions
Cells
July 6, 2023
Rogg M, Maier JI, Helmstädter M, Sammarco A, Kliewe F, Kretz O, Weißer L, Van Wymersch C, Findeisen K, Koessinger AL,
et al
.
RhoA
Levels of active (GTP bound)
RhoA
were determined by applying an
ELISA
-based
assay
kit (G-LISA,
BK124
;
Cytoskeleton Inc
., Denver, CO, USA).
Cell density
was equalized as described
for western blot
analysis.
(7)
Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome
Journal of translational medicine
June 18, 2023
Zhai R, Lenga Ma Bonda W, Leclaire C, Saint-Béat C, Theilliere C, Belville C, Coupet R, Blondonnet R, Bouvier D, Blanchon L,
et al
.
measurements
in vitro
RhoA activity was determined using a RhoA-specific G-LISA Activation
Assay
kit (
BK124
,
Cytoskeleton
, Denver, USA) following the per manufacturer’s protocol. The results were normalized to
(8)
The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancer
Molecular Medicine
June 14, 2023
Grace Velez Crespo, Jescelica Ortiz, Eliud Hernández O’Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia Kucheryavykh
h. Lysates were subjected to active
Cdc42
(#
BK127
,
Cytoskeleton
, Denver, CO, USA) or active
RhoA
(#
BK124
,
Cytoskeleton
, Denver, CO, USA) Glisa colorimetric
assay
as per manufacturer instructions and absorbance
(9)
Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice
Nature Communications
March 14, 2023
Qian Ye, Yi Liu, Guiji Zhang, Haijun Deng, Xiaojun Wang, Lin Tuo, Chang Chen, Xuanming Pan, Kang Wu, Jiangao Fan,
et al.
(
SEA523Hu
,
Cloud-Clone
Corp). Active RhoA was detected in a colorimetric RhoA activation assay (G-LISA) (
BK124
,
Cytoskeleton
, Denver, CO, USA).
Immunofluorescence
LX-2 cells were fixed in 4% formaldehyde for 25
(10)
Dual mechanism underlying failure of neural tube closure in the Zic2 mutant mouse
Disease Models & Mechanisms
March 1, 2023
Sarah Escuin, Saba Rose Rose Raza-Knight, Dawn Savery, Carles Gaston-Massuet, Gabriel L. Galea, Nicholas D. E. Greene, Andrew J. Copp
assay
and western blotting
Activated RhoA was determined using a RhoA G-LISA Activation
Assay
kit (
BK124
,
Cytoskeleton Inc
.) according to the manufacturer's instructions (Escuin et al., 2015). Total RhoA was
(11)
Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells
Open Biology
March 1, 2023
Jun Morishita, Paul Nurse
active RhoA-GTP levels,
assays
were performed using G-Lisa RhoA Activation kit (
Cytoskeleton
, cat. no.
BK124
.) according to manufacturer's instructions. This
assay
uses RhoA-GTP-binding proteins linked to the
(12)
PKCε inhibition prevents ischemia‑induced dendritic spine impairment in cultured primary neurons
Experimental and Therapeutic Medicine
February 16, 2023
Chenjie Ge, Xuefeng Wang, Yunhong Wang, Lilei Lei, Guohua Song, Mincai Qian, Shiliang Wang
assay
The rho-GTPase activity was measured using different G-LISA activation
assay
kits (cat. nos.
BK124
for active
RhoA
,
BK128
for active
Rac1
and
BK127
for
CDC42
;
Cytoskeleton, Inc
.) following the manufacturer's
(13)
Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling
Cancers
December 10, 2022
Yadav V, Jobe N, Satapathy SR, Mohapatra P, Andersson T.
BRAFi-sensitive and BRAFi-R
melanoma
cells was measured using a G-LISA RhoA activation assay kit (Cat#
BK124
,
Cytoskeleton Inc
., Denver, CO, USA) according to the manufacturer’s protocol. 2.8.
Immunofluorescence
(14)
p75NTR prevents the onset of cerebellar granule cell migration via RhoA activation
eLife
August 30, 2022
Juan P Zanin, Wilma J Friedman
for G-Lisa analysis according to the manufacturer’s instructions. G-Lisa for RhoA (
Cytoskeleton Inc
, #
BK124
).
Western blot
Cultured cells
were washed with ice-cold PBS and homogenized using 1% NP40, 1% Triton,
(15)
Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling
Frontiers in Immunology
August 18, 2022
Laura Keller et al.
agitation (400 rpm). To perform competition experiments using the G-LISA®
procedure
, we used G-LISA® RHOA (
BK124
) and RAC1 (
BK128
)
assays
(
Cytoskeleton
) according to the manufacturer’s instructions to assess if purified
(16)
Cannabinoid CB(1) receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice
Cell death & disease
July 7, 2022
Sánchez-de la Torre A, Aguado T, Huerga-Gómez A, Santamaría S, Gentile A, Chara JC, Matute C, Monory K, Mato S, Guzmán M,
et al
.
callosum extracts were processed for active RhoA quantification with the G-LISA kit (
Cytoskeleton
, Cat#
BK124
) following manufacturer’s guide. Behavioral assessments All tests were conducted during the light
(17)
Essential role of Rnd1 in innate immunity during viral and bacterial infections
Cell Death & Disease
June 2, 2022
Akhilesh Kumar, Shalabh Mishra, Ashish Kumar, Ashwin Ashok Raut, Seiichi Sato, Akinori Takaoka, Himanshu Kumar
Becton Dickinson
) secreted by the cells. RhoA activation was measured by RhoA G-LISA Activation
Assay
Kit (
BK124
,
Cytoskeleton, Inc
.) and IκBα phosphorylation was estimated using
PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit
(18)
The Hippo pathway mediates Semaphorin signaling
Science Advances
May 27, 2022
Zhipeng Meng et al.
the active RhoA/Rac1 interacting with Rho-GTP–binding strips using G-LISA kits (
Cytoskeleton Inc
. #
BK124
and #
BK127
). Briefly, 25 μg of cell lysates of each group was incubated with Rho-GTP–binding strips
(19)
PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor
Theranostics
May 9, 2022
Kankai Wang, Sishi Pan, Peiqi Zhao, Li Liu, Zhen Chen, Han Bao, Hao Wang, Ying Zhang, Qichuan Zhuge, Jianjing Yang
dephosphorylation function of PP2A, and its absorbance can be detected at 660 nm. G-LISA RhoA activation
assays
(
BK124
,
Cytoskeleton
, USA) are
ELISA
-based RhoA activation
assays
that can help
measure
RhoA activity in reprogrammed
(20)
Stress Elicits Contrasting Effects on Rac1-Cofilin Signaling in the Hippocampus and Amygdala
Frontiers in Molecular Neuroscience
May 3, 2022
Mihika Bose, Mohammad Sarfaraz Nawaz, Rakhi Pal, Sumantra Chattarji
no.
BK128
) and RhoA Activation Assay Biochem kit (
Cytoskeleton
Biochem kit; Denver, USA; catalog no.
BK124
) were used to
measure
the activity of Rac1 and RhoA respectively as per manufacturer’s protocol. The
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Reviews
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
RhoA G-LISA Activation Assay (absorbance) from Cytoskeleton, Inc.
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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